| Autor: |
Bravo, Marta Santos, Nicolás, David, Berengua, Carla, Fernandez, Mariana, Hurtado, Juan Carlos, Tortajada, Marta, Barroso, Sonia, Vilella, Anna, Mosquera, Maria Mar, Vila, Jordi, Marcos, María Angeles, Santos Bravo, Marta, Mosquera, Mar |
| Zdroj: |
Journal of Infectious Diseases; 10/15/2021, Vol. 224 Issue 8, p1325-1332, 8p |
| Abstrakt: |
Background: SARS-CoV-2 RT-PCR provides a highly variable cycle-threshold (Ct) value that cannot distinguish viral infectivity. Subgenomic RNA (sgRNA) has been used to monitor active replication. Given the importance of long RT-PCR positivity and the need for work reincorporation and discontinuing isolation, we studied the functionality of normalized viral loads (NVL) for patient monitoring and sgRNA for viral infectivity detection.Methods: NVL measured through the Nucleocapsid and RNA-dependent-RNA-polymerase genes and sgRNA RT-PCRs were performed in 2 consecutive swabs from 84 health-care workers.Results: NVL provided similar and accurate quantities of both genes of SARS-CoV-2 at two different time-points of infection, overcoming Ct-value and swab collection variability. Among SARS-CoV-2-positive samples, 51.19% were sgRNA-positive in the 1 stRT-PCR and 5.95% in the 2 ndRT-PCR. All sgRNA-positive samples had >4log10RNAcopies/1000cells, while samples with ≤1log10 NVL were sgRNA-negative. Although NVL were positive until 29 days after symptom onset, 84.1% of sgRNA-positive samples were from the first 7 days, which correlated with viral culture viability. Multivariate analyses showed that sgRNA, NVL and days of symptoms were significantly associated (p<0.001).Conclusions: NVL and sgRNA are two rapid accessible techniques that could be easily implemented in routine hospital practice providing a useful proxy for viral infectivity and COVID-19 patient follow-up. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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