| Autor: |
Shi, Yifeng, Ye, Lin, Shen, Shiwei, Qian, Tianchen, Pan, Youjin, Jiang, Yuhan, Lin, Jinghao, Liu, Chen, Wu, Yaosen, Wang, Xiangyang, Xu, Jiake, Jin, Haiming |
| Předmět: |
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| Zdroj: |
Phytotherapy Research; Oct2021, Vol. 35 Issue 10, p5694-5707, 14p |
| Abstrakt: |
Morin is a natural compound isolated from moraceae family members and has been reported to possess a range of pharmacological activities. However, the effects of morin on bone-associated disorders and the potential mechanism remain unknown. In this study, we investigated the anti-osteoclastogenic effect of morin in vitro and the potential therapeutic effects on ovariectomy (OVX)-induced osteoporosis in vivo. In vitro, by using a bone marrow macrophage-derived osteoclast culture system, we determined that morin attenuated receptor activator of nuclear factor (NF)-κB ligand (RANKL)-induced osteoclast formation via the inhibition of the mitogen-activated protein kinase (MAPK), NF-κB and calcium pathways. In addition, the subsequent expression of nuclear factor of activated T cells c1 (NFATc1) and c-fos was significantly suppressed by morin. In addition, NFATc1 downregulation led to the reduced expression of osteoclastogenesis-related marker genes, such as V-ATPase-d2 and Integrin β3. In vivo, results provided that morin could effectively attenuate OVX-induced bone loss in C57BL/6 mice. In conclusion, our results demonstrated that morin suppressed RANKL-induced osteoclastogenesis via the NF-κB, MAPK and calcium pathways, in addition, its function of preventing OVX-induced bone loss in vivo, which suggested that morin may be a potential therapeutic agent for postmenopausal osteoporosis treatment. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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