| Autor: |
Ye, Julia, Sheahon, Kathleen M., LeBoit, Philip E., McCalmont, Timothy H., Lang, Ursula E. |
| Předmět: |
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| Zdroj: |
Journal of Cutaneous Pathology; Nov2021, Vol. 48 Issue 11, p1353-1360, 8p, 3 Color Photographs, 2 Charts |
| Abstrakt: |
Background: BRCA1‐associated protein (BAP1) is a tumor suppressor whose loss is associated with various malignancies. The primary cilium is an organelle involved in signal transduction and cell cycle progression. Primary cilia have been shown to be absent in melanoma but retained to some extent in melanocytic nevi, and the severity of dysplasia influences the degree of cilia loss. Additionally, studies have revealed roles for BAP1 in centrosome and mitotic spindle formation. Because the primary cilium is nucleated on the mother centriole, we examined the connection between the presence of primary cilia and the formation of centrosomes in BAP1‐inactivated melanocytic tumors (BIMTs). Methods: We evaluated the cilia and centrosomes in 11 BIMTs and five conventional melanocytic nevi using immunofluorescence staining of acetylated alpha‐tubulin and gamma‐tubulin. Results: We found that, compared to nevi, BIMTs show loss of primary cilia and amplification of centrosomes. Occasional nevi also showed increased centrioles; however, these foci of amplification were more likely to be ciliated than those in BIMTs. Conclusions: Although centrosome amplification does not absolutely correlate with loss of primary cilia in melanocytic neoplasms, absence of BAP1 exacerbates the phenotype. Moreover, aberrant centrosome and cilia formation are likely critical in the pathogenesis of other BAP1‐inactivated tumors. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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