ANTAGONISTIC EFFECT OF LACTOBACILLUS ACIDOPHILUS AGAINST ENTEROPATHOGENS AND ITS UTILIZATION FOR DEVELOPMENT OF PROBIOTIC FOOD MIXTURES.

Autor: Sharon C. L., Usha V., Aneena E. R., Lakshmy P. S., Thomachan, Seeja
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Zdroj: Plant Archives (09725210); Oct2021, Vol. 21 Issue 2, p355-358, 4p
Abstrakt: Probiotics are live microbial supplement, which beneficially affect the host by improving the intestinal microbial balance. Lactobacillus acidophilus is one of the most common probiotic bacteria which have beneficial effects on the microbiota of the gastrointestinal tract. In the present study, L. acidophilus was used as the probiotic culture. The antagonistic effect of L. acidophilus against the enteropathogens strains of E. coli (MTCC 40), Salmonella enteritidis (MTCC 3219), Bacillus cereus (MTCC 430), Staphylococcus aureus (MTCC 430) and Shigella flexineri (MTCC 1457) was studied. The results showed that L.acidophilus was able to inhibit the growth of some of the selected pathogens in varying degrees. It was found to be most effective with a zone of inhibition of 24 mm recorded against Staphylococcus aureus. The antagonistic effect may be due to the production of organic acids, bacteriocins and hydrogen peroxide. Later, an attempt wasmade to develop probiotic food mixtures containing banana flour, soya flour, tomato, mango and papaya involving L acidophilus. The food mixture (25g) was mixed with 150ml water and stirred to obtain uniform slurry. Adjusted the pH to 4.5 and autoclaved at 121°C (1.5 kg cm-2) for 15 minutes. After cooling this was inoculated with 300µl (8.07log cfu ml-1) liquid culture of L. acidophilus (24 hour old culture) and incubated at 37° C for 24 hours. After fermentation it was freeze dried. The viability of the developed food mixtures were assessed for 6 months and it showed good viability which was within the recommended level of probiotic organism to assure health benefits. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index