A first-generation EST RH comparative map of the porcine and human genome.

Autor: Rink, Anette, Santschi, Elizabeth M., Eyer, Katie M., Roelofs, Benjamin, Hess, Markus, Godfrey, Myra, Karajusuf, Elif K., Yerle, Martine, Milan, Denis, Beattie, Craig W.
Předmět:
Zdroj: Mammalian Genome; Oct2002, Vol. 13 Issue 10, p578-587, 10p
Abstrakt: We have constructed a first-generation EST radiation hybrid comparative map of the porcine genome by assigning 1058 markers to the IMpRH7000 panel. Chromosomal localization was determined with a 2pt LOD of 4.8 for 984 markers, using the IMpRH mapping tool. Annotated ESTs represent 46.2% or 489 of the markers. Marker distribution was not stochastic and ranged from 0.41 for SSC8 to 1.77 for SSC12, respectively. Two hundred fifty-one markers assigned to the physical map of the pig did not find a homologous sequence in V22 of the human genome assembly, indicative of gaps in the assembled human genome sequence. The comparative porcine/human map covers 3290 MB, or 98.3% of the presumed size of the human genome. However, 60 breakpoints were identified between chromosomes, as well as 90 micro-rearrangements within synteny groups. Six porcine chromosomes—SSC2, 5, 6, 7, 12, and 14—correspond to the three gene-richest human chromosomes, HSA17, 19, and 22, and show above average marker density. Porcine Chrs 1, 8, 11, and X display a low DNA/marker ratio and correspond to the 'genome deserts' on HSA 18, 4, 13, and X. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index