| Autor: |
MAGWAZA, N. M., NXUMALO, E. N., MAMBA, B. B., NYONI, H., NTUSHELO, K., MSAGATI, T. A. M. |
| Předmět: |
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| Zdroj: |
Applied Ecology & Environmental Research; 2021, Vol. 19 Issue 5, p3505-3518, 14p |
| Abstrakt: |
This paper reports on a divergent approach employing isolation methods and high-resolution mass spectrometry to screen decomposition products that are a result of fungal action on biomass present in aquatic media. Fungi decompose organic matter in surface water through a process which plays a major role in nutrient cycling and maintenance of aquatic life. Moreover, the presence of pathogenic or potentially pathogenic species of fungi in drinking water distribution systems, may cause chronic diseases to consumers and these pathogenic fungi have the potential to produce a variety of secondary metabolites which play important roles in protecting the organism against competition for nutrients and survival. In this paper, the fungus Talaromyces flavus was isolated from Roodeplaat Dam in Gauteng province (South Africa) and was identified using sequence analysis of the internal transcriber spacer (ITS) region. Secondary metabolites produced by T. flavus grown in liquid culture were extracted and determined using Liquid chromatography-mass spectrometry (LC-MS) and comparative analysis of the secondary metabolite profile was done using Aspergillus fumigatus as a reference (ATCC 36607). The influence of nutrients, pH and incubation period on the production of secondary metabolites was demonstrated and validated statistically by principal component analysis (PCA). [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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