Biosurfactant Production by Bacillus tequilensis ZSB10: Structural Characterization, Physicochemical, and Antifungal Properties.

Autor: Cortés-Camargo, S., Acuña-Avila, P. E., Arrieta-Baez, D., Montañez-Barragan, B., Morato, A. I., Sanz-Martín, J. L., Barragan-Huerta, B. E.
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Zdroj: Journal of Surfactants & Detergents; Sep2021, Vol. 24 Issue 5, p773-782, 10p
Abstrakt: A new biosurfactant was obtained from a moderately halophilic bacterium identified as Bacillus tequilensis ZSB10 that was isolated from a saline water pond located in Tehuacan-Cuicatlan valley, Mexico. A kinetic analysis of the bacterial growth of the ZSB10 strain showed a maximum growth at 24 h regardless of the initial pH (5, 7.4, and 9). The best results were found at pH = 7.4 in terms of bacterial growth, besides which the produced biosurfactant showed emulsifying and surfactant properties with an emulsification index (E24) and surface tension change (ΔST) of 54 ± 0% and 26 mN m-1, respectively. Extracted ZSB10 crude biosurfactant had a yield of 106 ± 6 mg L-1, an E24 = 58.4 ±0.2%, and a ΔST = 26 mN m-1 with a critical micelle concentration (CMC) of 44.82 mg L-1. Also, its structure was characterized by MALDI-TOF mass spectrometry as a surfactin, iturin A, and fengycin mixture whose main isoform was leu/ile-7 C15 surfactin [M + Na]+. Finally, the ZSB10 crude biosurfactant showed antifungal activity against Helminthosporium sp., with a 79.3% growth inhibition and an IC50 of 1.37 mg per disc. Therefore, this biosurfactant could be used as biopesticide. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index