FML/QuilA-Vaccinated Dogs Naturally Infected with Leishmania infantum: Serum Cytokines, Clinicopathological Profile, and Parasitological Parameters.

Autor:	Almeida, Gregório Guilherme, Coura, Fernanda Morcatti, de Melo Barbieri, Jonata, Moura, Ana Carolina Junqueira, de Oliveira Paes-Leme, Fabiola, da Costa-Val, Adriane Pimenta
Předmět:	SKIN disease diagnosis COMMUNICABLE disease diagnosis TRANSMISSION of parasitic diseases CYTOKINES ANTIPARASITIC agents INTERLEUKINS REVERSE transcriptase polymerase chain reaction BIOCHEMISTRY IMMUNIZATION VIRAL load ANIMAL experimentation LEISHMANIA GLYCOSIDES PHENOMENOLOGY INFECTIOUS disease transmission MICROBIOLOGICAL techniques PARASITES POLYMERASE chain reaction BONE marrow BLOOD cell count DOGS BONE marrow examination
Zdroj:	BioMed Research International; 10/5/2021, p1-9, 9p
Abstrakt:	Dogs are the main reservoir of Leishmania infantum in endemic regions. Canine leishmaniasis, caused by L. infantum, can progress to a chronic disease resulting in death. Vaccines have been developed with a certain degree of success. The pathogenesis of this disease is not completely understood, especially in previously vaccinated dogs. We herein described clinical data, parasite load, serum levels of cytokines, and the reservoir potential in vdogs vaccinated with the fucose-mannose ligand (FML)/QuilA saponin vaccine (Leishmune™) naturally infected (Vi) and compared to vaccinated not infected dogs (Vn). Thirty-four dogs from private owners were divided into two groups: vaccinated/infected and vaccinated/uninfected. Clinical evaluation, hematological and biochemical parameters, and serum levels of cytokines were measured by conventional methods. The parasite burden in the bone marrow was measured by quantitative real-time PCR, and the transmissibility of parasites to sand flies was assessed by xenodiagnosis. Clinical, biochemical, and hematological parameters of vaccinated infected dogs were mostly normal. Vi dogs developed mild disease with low clinical scores. Serum levels of IL-10 were higher in Vi dogs, and a strong correlation was observed in IL-4 levels and the A/G ratio in Vi dogs. These results suggest a role of TH2 response in Vi dogs, although more data is needed to better understand the disease in vaccinated dogs. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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