| Abstrakt: |
To optimize the process of in vitro androgenesis, the influence of light intensity and quality on regeneration processes of the rice Oryza sativa L. callus was studied. The work was carried out based on plants of the Kaskad and Almaz varieties and the F2 hybrid Rassvet × Oxy 2x. To light calli on a regeneration medium, two types of LED lamps were used: with white light (color temperature 6500 K, which corresponds to the spectrum of midday sunlight) and violet light (emitting wavelengths of 400, 430, 660, and 730 nm). Experimental options were as follows: white light, 4200 lux; white + violet light in the 3/1 ratio, 3900 lux; violet light, 3600 lux; and intense white light, 7500 lux. The light intensity and quality equally affect the induction of morphogenic callus and the number of regenerants per callus. Spontaneous chromosome duplication occurs during the light stage of callus development. The quality of lighting has an ambiguous effect on the formation of doubled haploids of different genotypes. Intense white light contributes to the formation of highly ploid plants in all studied genotypes: 44.4–54.0% of doubled haploids and 1.2–38.9% of tetraploids. When being lighted using violet lamps, the Almaz variety and the hybrid produce more than 60.0% of doubled haploids, while the number of doubled haploids in the Kaskad variety decreases to 26.1–31.8%. To obtain a guaranteed high (49.4%) number of doubled haploids, it is advisable to use intense white type of lighting. [ABSTRACT FROM AUTHOR] |
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