| Autor: |
van den Berg, Tamar A. J., van den Heuvel, Marius C., Wiersema-Buist, Janneke, Adelmeijer, Jelle, Nieuwenhuijs-Moeke, Gertrude J., Lisman, Ton, Bakker, Stephan J. L., van Goor, Harry, TransplantLines Investigators, Annema-de Jong, J. H., Bakker, S. J. L., Berger, S. P., Blokzijl, J., Bodewes, F. A. J. A., de Boer, M. T., Damman, K., De Borst, M. H., Diepstra, A., Dijkstra, G., Douwes, R. M. |
| Předmět: |
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| Zdroj: |
Scientific Reports; 9/23/2021, Vol. 11 Issue 1, p1-11, 11p |
| Abstrakt: |
In kidney transplantation, microthrombi and fibrin deposition may lead to local perfusion disorders and subsequently poor initial graft function. Microthrombi are often regarded as donor-derived. However, the incidence, time of development, and potential difference between living donor kidneys (LDK) and deceased donor kidneys(DDK), remains unclear. Two open-needle biopsies, taken at preimplantation and after reperfusion, were obtained from 17 LDK and 28 DDK transplanted between 2005 and 2008. Paraffin-embedded sections were immunohistochemically stained with anti-fibrinogen antibody. Fibrin deposition intensity in peritubular capillaries(PTC) and glomeruli was categorized as negative, weak, moderate or strong and the number of microthrombi/mm2 was quantified. Reperfusion biopsies showed more fibrin deposition (20% to 100% moderate/strong, p < 0.001) and more microthrombi/mm2 (0.97 ± 1.12 vs. 0.28 ± 0.53, p < 0.01) than preimplantation biopsies. In addition, more microthrombi/mm2 (0.38 ± 0.61 vs. 0.09 ± 0.22, p = 0.02) and stronger fibrin intensity in glomeruli (28% vs. 0%, p < 0.01) and PTC (14% vs. 0%, p = 0.02) were observed in preimplantation DDK than LDK biopsies. After reperfusion, microthrombi/mm2 were comparable (p = 0.23) for LDK (0.09 ± 0.22 to 0.76 ± 0.49, p = 0.03) and DDK (0.38 ± 0.61 to 0.90 ± 1.11, p = 0.07). Upon reperfusion, there is an aggravation of microthrombus formation and fibrin deposition within the graft. The prominent increase of microthrombi in LDK indicates that they are not merely donor-derived. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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