| Autor: |
Ma, Guigen, Kuang, Yongjie, Lu, Zhenwan, Li, Xueqi, Xu, Ziyan, Ren, Bin, Zhou, Xueping, Zhou, Huanbin |
| Předmět: |
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| Zdroj: |
Journal of Integrative Plant Biology; Sep2021, Vol. 63 Issue 9, p1606-1610, 5p |
| Abstrakt: |
Streptococcus canis Cas9 (ScCas9) is an RNA‐guided endonuclease with NNG protospacer adjacent motif (PAM) specificity whose genome‐editing activity in rice is locus‐dependent. Here we investigated the performance of a ScCas9 variant named Sc++ at different NNG PAM sites in the rice genome; Sc++ harbors a T1227K mutation and the substitution of a positively charged loop (residues 367–376). Sc++ nuclease achieved broader genome editing compared to the original ScCas9, and its nickase improved targeted base editing in transgenic rice plants. Using the high‐efficiency adenine base editor rBE73b, we generated many new OsGS1 alleles suitable for screening of rice germplasm for potential herbicide resistance in the future. The CRISPR/Sc++ system expands the genome‐editing toolkit for rice. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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