Bending the MDCK cell primary cilium increases intracellular calcium.
| Autor: | Praetorius, H.A., Spring, K.R. |
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| Předmět: |
FLUORESCENCE microscopy
SOLUTION (Chemistry) SOLID solutions CALCIUM MICROSCOPY SEMICONDUCTOR doping CALCIUM metabolism CELL metabolism CALCIUM antagonists ALCOHOLS (Chemical class) ANIMALS BIOLOGICAL transport CELL lines CELL physiology CELLULAR signal transduction DOGS ENZYME inhibitors EPITHELIAL cells KIDNEY tubules PYRIDINE VERAPAMIL PHYSIOLOGIC strain FLUORESCENT dyes PHARMACODYNAMICS |
| Zdroj: | Journal of Membrane Biology; Nov2001, Vol. 184 Issue 1, p71-79, 9p |
| Abstrakt: | We tested the hypothesis that the primary cilium of renal epithelia is mechanically sensitive and serves as a flow sensor in MDCK cells using differential interference contrast and fluorescence microscopy. Bending the cilium, either by suction with a micropipette or by increasing the flow rate of perfusate, causes intracellular calcium to substantially increase as indicated by the fluorescent indicator, Fluo-4. This calcium signal is initiated by Ca2+-influx through mechanically sensitive channels that probably reside in the cilium or its base. The influx is followed by calcium release from IP3-sensitive stores. The calcium signal then spreads as a wave from the perturbed cell to its neighbors by diffusion of a second messenger through gap junctions. This spreading of the calcium wave points to flow sensing as a coordinated event within the tissue, rather than an isolated phenomenon in a single cell. Measurement of the membrane potential difference by microelectrode during perfusate flow reveals a profound hyperpolarization during the period of elevated intracellular calcium. We conclude that the primary cilium in MDCK cells is mechanically sensitive and responds to flow by greatly increasing intracellular calcium. [ABSTRACT FROM AUTHOR] |
| Databáze: | Complementary Index |
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