| Autor: |
Smith, Ignacio, Mc Callum, Gregorio Juan, Sabljic, Adriana Victoria, Marfia, Juan Ignacio, Bombicino, Silvina Sonia, Trabucchi, Aldana, Iacono, Ruben Francisco, Birenbaum, Joaquín Manuel, Vazquez, Susana Claudia, Minoia, Juan Mauricio, Cascone, Osvaldo, López, María Gabriela, Taboga, Oscar, Targovnik, Alexandra Marisa, Wolman, Federico Javier, Fingermann, Matías, Alonso, Leonardo Gabriel, Valdez, Silvina Noemí, Miranda, María Victoria |
| Zdroj: |
Biotechnology & Bioengineering; Oct2021, Vol. 118 Issue 10, p4129-4137, 9p |
| Abstrakt: |
Serology testing for COVID‐19 is important in evaluating active immune response against SARS‐CoV‐2, studying the antibody kinetics, and monitoring reinfections with genetic variants and new virus strains, in particular, the duration of antibodies in virus‐exposed individuals and vaccine‐mediated immunity. In this study, recombinant S protein of SARS‐CoV‐2 was expressed in Rachiplusia nu, an important agronomic plague. One gram of insect larvae produces an amount of S protein sufficient for 150 determinations in the ELISA method herein developed. We established a rapid production process for SARS‐CoV‐2 S protein that showed immunoreactivity for anti‐SARS‐CoV‐2 antibodies and was used as a single antigen for developing the ELISA method with high sensitivity (96.2%) and specificity (98.8%). Our findings provide an efficient and cost‐effective platform for large‐scale S protein production, and the scale‐up is linear, thus avoiding the use of complex equipment like bioreactors. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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