SYNTHESIS OF TIO2 NPS BY BACILLUS CEREUS ISOLATED FROM SOIL AND EVALUATION OF THEIR ACTIVITY AGAINST SOME PATHOGENIC BACTERIA ISOLATED FROM DIARRHEA.

Autor: Daham, Shaymaa Naji
Předmět:
Zdroj: Biochemical & Cellular Archives; 2021Supplement1, Vol. 21, p2145-2152, 8p
Abstrakt: The nanoparticle was synthesized from TiO2 by employing Bacillus cereus. The bacterial isolates were isolated from soil and identified according to morphological and physiological characteristic. B. cereus exhibits a potential activity to produce nanoparticles (NPs) from titanium dioxide (TiO2 ). UV- visible spectroscopy result exhibited absorbance peak of TiO2 NP at ~ 560 nm. Scanning electron microscope (SEM) was exhibit the size and shape of the particles. The result revealed that the particles were in the nano range, and the outer surface were downy and showing mono dispersity, these characteristic were confirmed by AFM. The formation of nanoparticles X-ray diffraction (XRD) technique was used to characterize the particle size and morphology. The effectiveness of TiO2 NP as antibacterial agent was tested against some pathogenic bacteria isolated from diarrhea. The results showed that the highest inhibition zone diameter at 100% concentration were (15, 12, 14, 17, 16, 13 mm) for Serratia marcescencens, Proteus mirabilis, Enterobacter aerogenes, Salmonella typhi, Klebsiella pneumonia and Hafnia alvei, respectively while the lowest inhibitory diameter was 8 mm in 25% concentration against proteus mirabilis. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index