| Abstrakt: |
The infection of the Urinary tract (UTI) represents the most health problem in the world affecting millions of people every year. E. coli is the most bacterium that causes urinary tract infections in humans. E. coli can because transitory damage of DNA followed by incomplete DNA repair during cell division, so this finally leading to chromosome deflections. Samples of urines were gained from 58 patients with urinary tract infection (UTI) and diagnosed as infection with E. coli, also 35 control samples (noninfected patients). The samples undergrow to micronucleus assay in renal epithelial cells. Frequencies of cells with micronucleated (MN), binucleated (BN) Condensed chromatin (CC), karyorrhexis (KR), pyknosis (PN), karyolysis cells (KL) were computed in all groups (patients and controls) as parameters for the evaluation of the stability of the genome. So, the aim of this study to investigate the possibility of usage of renal epithelial cells from the urinary tract to identifying the genotoxicity and DNA damage effects of UTI patients. The present study showed that the micronucleus (MN) and karyorrhexis (KR) were higher percentages in males more than females in patients with UTI compared with healthy controls as 4.22%, 3.91%, respectively while in females as 3.86%, 2.97%, respectively. In micronucleus obtain p-value as (p>0,05). In our study observed that the E. coli associated with urinary tract infection can induce DNA damage for epithelial cells. So can be suggested if the patients administrated antibiotics in early infection, the level of DNA damage can be reduced. [ABSTRACT FROM AUTHOR] |
