| Autor: |
Cotton, D.W.K., van den Hurk, José J.M.A., Mier, P.D. |
| Předmět: |
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| Zdroj: |
British Journal of Dermatology; Jun1973, Vol. 88 Issue 6, p579-582, 4p |
| Abstrakt: |
The hydrolysis of acetylcholine by preparations of mouse and human skin has been studied. Activity has been demonstrated in both soluble and particulate fractions. In the former case at least two enzymes appear to be present; the Km values suggest these to be ‘true’ (3.1.1.7) and ‘pseudo’ cholinesterase (3.1.1.8). Biopsy specimens of skin from normal and atopic subjects yielded similar kinetic data. This supports our previous conclusion that a reduced rate of hydrolysis of acetylcholine is not associated with the atopic disposition. In our previous communication (Cotton, van den Hurk & Mier, 1973) we described the measurement of carboxylic ester hydrolases (including cholinesterases) using the synthetic substrate β-naphthyl acetate. No differences were found between normal and atopic subjects; however, the limitations inherent in this approach were discussed. We now wish to report an extension of this work. The use of the physiological substrate, acetylcholine, has overcome possible objections concerning the specificity of the assay technique; in addition, by employing a radiometric rather than an optical assay, it has proved possible to examine both the soluble and the particle-bound cholinesterase activity of skin. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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