| Abstrakt: |
The aim of this study was to examine optimal induction method for carpospore release from Agarophyton vermiculophyllum cystocarps for seedling production. We tested the effects of environmental factors on carpospore release by using five different induction methods; spontaneous, desiccation, low temperature, desiccation+low temperature, and osmotic shock. Also, carpospores release was estimated at three temperatures (20, 25, and 30°C), and then under combinations of three day lengths (8, 12, and 16h) and two irradiances (30 and 60 μmol photons m-2 s-1), after pretreatment at desiccation+low temperature for 2 hr. The number of carpospores released was between 113 ~ 682 spores/cystocarp/day and it was maximal in the desiccation+low temperature treatment. Optimal environmental conditions for carpospore release of A. vermiculophyllum were 25°C, 16 h, and 60 μmol photons m-2 s-1. The present results suggest that massive carpospores for seedling production of A. vermiculophyllum could be obtained under a combination of 25°C, 16 h, and 60 μmol photons m-2 s-1 after pretreatment in the desiccation+low temperature. [ABSTRACT FROM AUTHOR] |
