| Autor: |
Turnbaugh, Carter, Axelrod, Jeremy J., Campbell, Sara L., Dioquino, Jeske Y., Petrov, Petar N., Remis, Jonathan, Schwartz, Osip, Yu, Zanlin, Cheng, Yifan, Glaeser, Robert M., Mueller, Holger |
| Předmět: |
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| Zdroj: |
Review of Scientific Instruments; May2021, Vol. 92 Issue 5, p1-9, 9p |
| Abstrakt: |
Transmission electron microscopy (TEM) of vitrified biological macromolecules (cryo-EM) is limited by the weak phase contrast signal that is available from such samples. Using a phase plate would thus substantially improve the signal-to-noise ratio. We have previously demonstrated the use of a high-power Fabry–Perot cavity as a phase plate for TEM. We now report improvements to our laser cavity that allow us to achieve record continuous wave intensities of over 450 GW/cm2, sufficient to produce the optimal 90° phase shift for 300 keV electrons. In addition, we have performed the first cryo-EM reconstruction using a laser phase plate, demonstrating that the stability of this laser phase plate is sufficient for use during standard cryo-EM data collection. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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