DNA Duplexes Containing 2′-Deoxy-2′-Iodoacetamidouridine as Reagents for Affinity Modification of Proteins.

Autor:	Borisova, O. A., Romanenkov, A. S., Metelev, V. G., Kubareva, E. A., Zubin, E. M., Timchenko, M. A., Oretskaya, T. S.
Předmět:	PROTEINS DNA CARRIER proteins THYMIDINE AMINO acids TRANSCRIPTION factors
Zdroj:	Molecular Biology; May/Jun2003, Vol. 37 Issue 3, p456-463, 8p
Abstrakt:	DNA duplexes containing the iodoacetamido group at position 2′ of the ribose moiety were proposed for affinity modification of Cys in DNA-binding proteins. Reactive DNA derivatives were obtained with iodoacetic anhydride and synthetic oligodeoxyribonucleotides containing 2′-amino-2′-deoxyuridine in place of thymidine at various positions. The derivatives were tested for reaction with amino acids and peptides and shown to specifically interact with Cys-containing proteins. The possibility of using the modified DNA duplexes to probe the protein SH group close to the DNA sugar-phosphate backbone in DNA–protein complexes was demonstrated with the example of subunit p50 of human transcription factor NF-κB. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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