Cancer stemness of CD10‐positive cells regulated by Hedgehog pathway promotes the resistance to cisplatin in oral squamous cell carcinoma.

Autor:	Wang, Yifei, Li, Qingxiang, Xu, Le, Chen, Junpeng, Pu, Yinfei, Wang, Lin, Sun, Hongfang, Guo, Yuxing, Guo, Chuanbin
Předmět:	BIOCHEMISTRY REVERSE transcriptase polymerase chain reaction MOUTH tumors B cell lymphoma QUANTITATIVE research METASTASIS PHENOMENOLOGY CELL survival EPITHELIAL-mesenchymal transition COMPARATIVE studies CISPLATIN HEDGEHOG signaling proteins STEM cells DESCRIPTIVE statistics CELL lines POLYMERASE chain reaction SQUAMOUS cell carcinoma DRUG resistance in cancer cells
Zdroj:	Oral Diseases; Sep2021, Vol. 27 Issue 6, p1403-1411, 9p, 2 Color Photographs, 2 Graphs
Abstrakt:	Objective: To explore the role of CD10 in cisplatin resistance of oral squamous cell carcinoma (OSCC) and its association with the Hedgehog (Hh) signaling pathway and cancer stem cells (CSCs). Methods: The correlation between cell viability and CD10 expression was analyzed in different OSCC cell lines after the cisplatin treatment. Genes related to chemotherapy resistance, cancer stem cells and the epithelial–mesenchymal transition were detected by quantitative real‐time PCR (qPCR) in CD10high and CD10low OSCC cells. Mouse xenograft model and venous metastasis model were used to explore the potential regulatory mechanism of the resistance effect of CD10 on cisplatin. Results: The higher expression of CD10 gene in different cell lines displayed enhanced cisplatin resistance ability. The expression of genes related to chemotherapy resistance, cell stemness, and the epithelial–mesenchymal transition was significantly higher in CD10high cells compared with CD10low cells. Moreover, the combination of cisplatin and Hh pathway inhibitors significantly reduced the resistance of CD10 to cisplatin in the xenograft model and venous metastasis models. Conclusion: CD10‐positive cells are implicated in developing cisplatin resistance of OSCC, which could be related to its cancer stem cell characteristics regulated by the Hedgehog pathway. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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