Autor: |
Mohammed, Sarmad Jassem, Al-Watifi, Noora Amir, Jameel, Zahraa Isam, Mohammed, Amera Kamal, Alasadi, Yusor Fadhil |
Předmět: |
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Zdroj: |
Indian Journal of Forensic Medicine & Toxicology; Jul-Sep2021, Vol. 15 Issue 3, p5110-5115, 6p |
Abstrakt: |
Cryptosporidium is an apicomplexan parasite that is increasingly recognized among immunocompetent hosts as a leading cause of childhood diarrhea in lower source settings and of waterborne diarrheal outbreaks in high income countries. C. parvum, which infects bovines, wild animals and humans, account for the majority of human infections. Cryptosporidium oocysts are transmitted via the fecal oral route, including by person to person spread, from contaminated food or water, or from contact with infected animals. 300 Stool samples were collected from patients in Hilla city from different hospitals. DNA extracted from stool samples, PCR amplification of DNA. Genotyping of HSP70 gene and DHFR Gene was performed using a polymerase chain reaction technique, followed by DNA sequencing. Accordingly, these DNA polymorphisms were confirmed using DNA sequencing. The sequencing results showed the presence of many SNPs determined in the HSP70 gene and DHFR gene. [ABSTRACT FROM AUTHOR] |
Databáze: |
Complementary Index |
Externí odkaz: |
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