| Autor: |
Aghaizu, Nozie D., Warre-Cornish, Katherine M., Robinson, Martha R., Waldron, Paul V., Maswood, Ryea N., Smith, Alexander J., Ali, Robin R., Pearson, Rachael A. |
| Zdroj: |
Cell Reports; Aug2021, Vol. 36 Issue 5, pN.PAG-N.PAG, 1p |
| Abstrakt: |
In development, almost all stratified neurons must migrate from their birthplace to the appropriate neural layer. Photoreceptors reside in the most apical layer of the retina, near their place of birth. Whether photoreceptors require migratory events for fine-positioning and/or retention within this layer is not well understood. Here, we show that photoreceptor nuclei of the developing mouse retina cyclically exhibit rapid, dynein-1-dependent translocation toward the apical surface, before moving more slowly in the basal direction, likely due to passive displacement by neighboring retinal nuclei. Attenuating dynein 1 function in rod photoreceptors results in their ectopic basal displacement into the outer plexiform layer and inner nuclear layer. Synapse formation is also compromised in these displaced cells. We propose that repeated, apically directed nuclear translocation events are necessary to ensure retention of post-mitotic photoreceptors within the emerging outer nuclear layer during retinogenesis, which is critical for correct neuronal lamination. [Display omitted] • Photoreceptor nuclei exhibit apico-basal motility in the developing mouse retina • Rapid apically directed nuclear translocation is powered by dynein 1 • Dynein 1 disruption impedes rapid apical nuclear translocation, causing displacement • Synapse formation is disrupted in basally displaced photoreceptors Photoreceptors occupy a defined layer in the retina. Aghaizu et al. show that mammalian rod and cone photoreceptors use repeated, dynein-1-driven apically directed nuclear translocations for fine-positioning and retention within this layer. Dynein 1 disruption in photoreceptors results in ectopic displacement beyond the photoreceptor layer and impaired synapse formation. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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