| Abstrakt: |
[Objectives] The anti-tumor, anti-bacterial, anti-acetylcholinesterase and anti-α-glucosidase activity in vitro of five chromenes isolated from Helianthus annuus disk was studied, in order to provide reference for the development and utilization of H. annuus disk resources. [Methods] The effect of different concentrations of chromenes on the survival rate of leukemia HL-60 cells, lung cancer A549 cells, liver cancer SMMC-7721 cells, breast cancer MCF-7 cells and colon cancer SW480 cells was detected by MTS method, and the IC50 was calculated. The inhibitory activity of chromenes against Escherichia coli, Staphylococcus aureus subsp. aureus, Salmonella enterica subsp. enterica, Pseudomonas aeruginosa and Candida albicans was detected by microdilution method. The DTNB substrate method was used to detect the inhibitory activity of chromenes on acetylcholine. The PNPG substrate method was used to detect the inhibitory activity of chromenes on α-glucosidase. [Results] The five chromenes had no obvious in vitro inhibitory activity on the five kinds of tumor cells, with IC50 greater than 40 μM. The five chromenes had no obvious in vitro inhibitory activity against the four kinds of bacteria and C. albicans. The five chromenes had certain inhibitory activity on acetylcholinesterase, and among them, 6-acetyl-2,2-dimethylchromene and 6-acetyl-7-hydroxy-2,3-dimethylchromene showed strong inhibitory activity on acetylcholinesterase, with IC50 of 28.253 and 16.945 μM, respectively, both smaller than that（0.275 μM） of the positive control tacrine（P<0.01）. The five chromenes showed good inhibitory effect on α-glucosidase, and among them, 7-hydroxy-6-hydroxyacetyl-2,2-dimethylchromene and 6-acetyl-7-hydroxy-2,3-dimethylchromene had stronger inhibitory activity, with IC50 of 20.240 and 21.052 μM, respectively, significantly better than that（169.780 μM） of the positive control acarbose（P<0.01）. [Conclusions] The five chromenes in H. annuus disk have certain in vitro inhibitory activity against acetylcholinesterase and α-glucosidase and certain potential in fighting neurodegenerative diseases and diabetes. [ABSTRACT FROM AUTHOR] |
