Multinational evaluation of the BioFire® FilmArray® Pneumonia plus Panel as compared to standard of care testing.

Autor: Ginocchio, Christine C., Garcia-Mondragon, Carolina, Mauerhofer, Barbara, Rindlisbacher, Cory, and the EME Evaluation Program Collaborative, Forcelledo, L., Fernández, J., Lienhard, R., Kerschner, H., Rossolini, G. M., Armand-Lefèvre, L., d'Humières, C., Cambau, E., Benmansour, H., Cavallo, R., Altwegg, M., Berlinger, L., Bonnet, R., Saint-Sardos, P., Meex, C.
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Zdroj: European Journal of Clinical Microbiology & Infectious Diseases; Aug2021, Vol. 40 Issue 8, p1609-1622, 14p
Abstrakt: This study compared standard of care testing (SOC) to BioFire® FilmArray® Pneumonia plus Panel (PNplus). PNplus detects 15 bacteria with semiquantitative log bin values, 7 antibiotic resistance markers, three atypical bacteria (AB), and eight viral classes directly from bronchoalveolar lavage-like specimens (BLS) and sputum-like specimens (SLS). Fifty-two laboratories from 13 European countries and Israel tested 1234 BLS and 1242 SLS with PNplus and SOC. Detection rates and number of pathogens/samples were compared for PNplus pathogens. PNplus bin values and SOC quantities were compared. Three thousand two hundred sixty-two bacteria in PNplus were detected by PNplus and/or SOC. SOC detected 57.1% compared to 95.8% for PNplus (p ≤ 0.0001). PNplus semiquantitative bin values were less than SOC, equal to SOC, or greater than SOC in 5.1%, 25.4%, and 69.6% of results, respectively. PNplus bin values were on average ≥ 1 log than SOC values (58.5% 1–2 logs; 11.0% 3–4 logs). PNplus identified 98.2% of MRSA and SOC 55.6%. SOC detected 73/103 AB (70.9%) and 134/631 viruses (21.2%). PNplus detected 93/103 AB (90.3%) and 618/631 viruses (97.9%) (p ≤ 0.0001). PNplus and SOC mean number of pathogens/samples were 1.99 and 1.44, respectively. All gram-negative resistance markers were detected. PNplus and SOC results were fully or partially concordant for 49.1% and 26.4% of specimens, respectively. PNplus was highly sensitive and detected more potential pneumonia pathogens than SOC. Semiquantification may assist in understanding pathogen significance. As PNplus generates results in approximately 1 h, PNplus has potential to direct antimicrobial therapy in near real time and improve antimicrobial stewardship and patient outcomes. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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