| Autor: |
Neha, S.L., Pannu, Arzoo, Sahoo, Pravat Kumar |
| Předmět: |
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| Zdroj: |
Analytical Chemistry Letters; Aug2021, Vol. 11 Issue 4, p553-562, 10p |
| Abstrakt: |
The easy, rapid, accurate, and specific demonstrating UV spectroscopic process for validation of Dopamine in pure bulk and nanoformulation has been developed. Dopamine works as a precursor in a biosynthetic pathway of epinephrine and nor-epinephrine. Mainly it is a neurotransmitter in the central nervous systems and peripheral nervous systems to convey the signals from one neuron to another neuron as a biological messenger. The deficiency of DOPA in cells causes Parkinson's disease. The major aim in the therapy of Parkinson's disease is to increase the normal concentration of dopamine in the brain but dopamine is unable to reach the brain owing to the inability of dopamine to cross the blood brain barrier hence NLC of DOPA is a new approach of drug delivery without any modification to the drug molecule. There is no specific UV spectroscopy technique that has been described for the validation and assessment of DOPA in bulk to prepare dopamine loaded NLC formulation (NLC-DOPA). Current work has shown the drug characterization to confirm the identification and compatibility of the drug with lipids by the DSC and FTIR. The UV Spectroscopy technique has been developed and validated to determine dopamine in bulk and NLC-DOPA formulation as continued via the International Conference of Harmonization guidelines. The developed method follows the beer's law in a series of 10-70 μg/ml along with a correlation coefficient of R2 = 0.999 at the wavelength of 280 nm. The % Assay of dopamine was found to be near to 100 %. The % recovery of developed method was found to be accurate in the form of Mean ± %RSD and the method was found to be precise at differential day variation (Mean ± % RSD). The LOD and LOQ were performed appropriately. This method has been productively used to find out the amount of dopamine hydrochloride available in the formulation. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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