| Autor: |
Taha, Dalia A., Mahfouz, Eman R., Bibars, Mona A., Hassan, Nagwa A., Othman, Othman E. |
| Předmět: |
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| Zdroj: |
Jordan Journal of Biological Sciences; Jun2021, Vol. 14 Issue 2, p245-251, 7p |
| Abstrakt: |
Fertility deficiency is one of the major causes which lead to a decrease in river buffalo’s production that has great economic importance in Egypt. Infection of the endometrium with different types of bacteria leads to inflammations causing infertility in severe conditions. The difference in cytokines expression profiles has a role in the early detection of subclinical endometritis-infected buffalo. The present study aimed to assess some cytokine gene expressions in endometritis-infected buffaloes using RT-qPCR. The uteri samples were collected from 60 animals with inflammation signs and other 60 healthy animals. RNA was extracted from uteri samples and cDNA was synthesized from extracted RNA. Gene expressions were detected by real-time PCR using SYBR Green PCR Master-Mix and primers specific for tested genes (IL-1α, IL-1β, IL-6, IL- 10 and TNF-α). Cycle threshold (Ct) mean values of triplicate samples were utilized for analysis. Chi-square test was utilized to determine the significant differences (P<0.05) in gene expression of tested genes using GAPDH as a house-keeping gene for normalization. Bacterial examination of uteri from infected buffaloes showed the presence of bacterial contamination with Escherichia coli, Klebsiella pneumonia and Proteus vulgaris, whereas the uteri of apparently-healthy animals did not show any of the previous pathogenic bacteria. The RT-qPCR results revealed that the gene expression of three tested cytokines; IL-1α. IL-1β and IL-6increased in endometritis-infected buffaloes with 1.3, 1.7 and 5 folds, respectively compared to their expression in healthy animals. On the contrary, the expressions of two other genes: IL-10 and TNF-α showed down regulations with 0.2 and 0.4 folds, respectively in infected buffaloes when compared with those in uninfected animals. It can be concluded that the tested cytokine genes in endometritis-infected buffaloes showed different responses to the endometritis infection, where three of them: IL-1α. IL-1β and IL-6 were up-regulated, whereas IL-10 and TNF-α were downregulated with different levels of significant values. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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