| Abstrakt: |
Objective To investigate the changes of appetite regulating factors and their receptors in hypothalamus of rats with chronic mild unpredictable stress depression, and explore the antidepressant mechanism of Xiaoyao San. Methods The depression rat model was established with stimulation of chronic unpredictable mild stress factors, and they were randomly divided into the model group, Xiaoyao San group and fluoxetine group (n = 10), with 10 normal rats as the normal group. After the intervention of Xiaoyao San for 4 weeks, the behavior of the model rats was verified by Sugar Water Consumption Test and Feeding Behavior Test. The changes of Orexin A, Orexin B, Orexin R1, Orexin R2, leptin and leptin receptor (LEPR) were detected by immunohistochemistry. Results Compared with the normal group, 1 % sucrose water consumption, sugar water consumption rate, body weight and food intake in the model group were decreased (P <0. 01), and the latency of approach to food was prolonged ( P < 0.05). In hypothalamic arcuate nucleus (ARC), the expression of Orexin A increased ( P <0. 01), while the expression of Orexin B and leptin decreased ( P < 0. 05, P < 0. 01 ) in the model group compared with the normal group. In Paraventricular Hypothalamic Nucleus (PVN), the expression of Orexin A increased(P <0. 01), while the expression of Orexin R1 and Orexin R2 decreased(P <0. 05), and the expression of leptin and its receptor LEPR decreased(P < 0. 05, P <0. 01 ) in the model group compared with the normal group. Xiaoyao San showed an up-regulation effect on sugar consumption, body weight, and food intake in depression model rats ( P < 0. 05 ), and also had a regulatory effect on the latency of approach to food and the expression of Orexin A, Orexin R1 and leptin in hypothalamus ( P <0. 05, P <0. 01 ) . Fluoxetine increased the expression of Orexin B and Orexin R2 (P < 0. 05, P < 0.01). Conclusion Xiaoyao San seems to have an antidepressant effect by regulating the levels of Orexins and leptin in the central nervous system, and appetite regulating factors may be one of its main targets for antidepressant. [ABSTRACT FROM AUTHOR] |
