| Autor: |
Shojiro KATOH, RAO, Srinivas K., SURYAPRAKASH, Vaddi, Akio HORIGUCHI, Toshihiro KUSHIBIKI, Kenichiro OJIMA, Masayuki SHINCHI, Masaru IWASAKI, Masayuki TAKEDA, SENTHILKUMAR, Rajappa, RAJMOHAN, Mathaiyan, KARTHICK, Ramalingam, PREETHY, Senthilkumar, ABRAHAM, Samuel J. K. |
| Předmět: |
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| Zdroj: |
Tokai Journal of Experimental & Clinical Medicine; Apr2021, Vol. 46 Issue 1, p1-6, 6p |
| Abstrakt: |
Background: Buccal mucosal epithelial cells show promising application for various regenerative medicine approaches. In this study, we examined the feasibility of culturing rabbit and human buccal mucosal epithelial cells in a novel thermoreversible gelation polymer (TGP) scaffold, without feeder layers or other foreign proteins. Methods & Results: The results of this 28-day in vitro culture, u sing the conventional technique (2D) and TGP (3D) showed that the epithelial cell morphology could be maintained only in the TGP group while cells in the 2D group de-differentiated to fibroblast morphology in both human and rabbit samples. CK3 expression, a marker for epithelial differentiation was higher in 3D-TGP cultured cells than 2D. Conclusion: TGP based in vitro cell culture is a prospective methodology to culture buccal mucosal epithelial cells efficiently without using foreign biological components for tissue engineering applications. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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