| Autor: |
Kovganko, N. V., Kashkan, Zh. N., Krivenok, S. N., Potapovich, M. V., Eremin, A. N., Metelitsa, D. I. |
| Předmět: |
|
| Zdroj: |
Chemistry of Natural Compounds; Jan/Feb2004, Vol. 40 Issue 1, p71-74, 4p |
| Abstrakt: |
The kinetics of catalase decomposition of H2O2 (50 mM) inhibited by the flavonolic glycoside astragalin (1) from the aerial part of Gymnocarpium dryopteris were studied. 1 (0.3-30 M) is a strong inhibitor of the catalase process and was tested as a catalase (CAT) protector from ultrasonic activation at 45 °C by treatment of enzyme solutions (1.2 nM) in phosphate buffer(7.5 mM) at pH 5.5 with low-frequency ultrasound (US. 27 kHz) at initial specific power 60 W/cm². 1 inhibited US-inactivation of CAT according to the rate constants for US-inactivation of CAT in the presence of 1 with and without inhibitor. 1 at concentrations of 1.0 and 20.0 µM decreases US-inactivation up to 22.3 and 19.5% of the initial level. The useful properties and effectiveness of 1 are better than the synthetic inhibitors-antioxidants propylgallate and 4-t-butylpyrocatechin. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
