| Autor: |
Witkowska, Agata, Heinz, Leonard P., Grubmüller, Helmut, Jahn, Reinhard |
| Předmět: |
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| Zdroj: |
Nature Communications; 6/14/2021, Vol. 12 Issue 1, p1-7, 7p |
| Abstrakt: |
Membrane fusion is fundamental to biological processes as diverse as membrane trafficking or viral infection. Proteins catalyzing membrane fusion need to overcome energy barriers to induce intermediate steps in which the integrity of bilayers is lost. Here, we investigate the structural features of tightly docked intermediates preceding hemifusion. Using lipid vesicles in which progression to hemifusion is arrested, we show that the metastable intermediate does not require but is enhanced by divalent cations and is characterized by the absence of proteins and local membrane thickening. Molecular dynamics simulations reveal that thickening is due to profound lipid rearrangements induced by dehydration of the membrane surface. Proteins need to overcome energy barriers to induce intermediate steps in membrane fusion. Using lipid vesicles in which progression to hemifusion is arrested, the authors show that the metastable intermediate is enhanced by divalent cations and is characterized by the absence of proteins and local membrane thickening. Simulations reveal that thickening is induced by dehydration of the membrane surface. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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