| Autor: |
Widjiastuti, Ira, Mudjiono, Moch., Sudiartha, Nancy Cynthia, Samuel, Grace Angelina, Sawitri Mega, Ni Putu |
| Předmět: |
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| Zdroj: |
Indian Journal of Forensic Medicine & Toxicology; Apr-Jun2021, Vol. 15 Issue 2, p2597-2605, 9p |
| Abstrakt: |
Objective: Calcium hydroxide is widely used as pulp-capping material, but it is resistant to Aggregatibacter actinomycetemcomitans bacteria. Because of this deficiency, many researchers are considering using alternative natural ingredients, such as propolis. Propolis has been widely used in dentistry due to its properties and because it has active ingredients that can act as antibacterial agents. It is hoped that when calcium hydroxide and propolis are combined, the combination can overcome the disadvantages. The aim of this study was to determine the dosage effectiveness of the combination calcium hydroxide-propolis as an inhibitor for A. actinomycetemcomitans biofilm. Material and methods: The sample was divided into five groups. The positive control group is a combination of calcium hydroxide-sterile aquadest, and the negative control group is A. actinomycetemcomitans bacteria. Group I is a combination of calcium hydroxidepropolis with a ratio of 1:1, group II has a ratio of 1:1.5, and group III has a ratio of 1:2. Each group consists of five replications. Combination of calcium hydroxide-propolis and A. actinomycetemcomitans bacterial suspension was incubated for 2x24 hours then washed three times using PBS. Furthermore, 2% of crystal violet was used for staining and covered with aluminum foil and then incubated for 15 minutes. Isopropanol 200µL was added, followed by a measurement of OD with an ELISA reader, and the OD values were calculated using an inhibition biofilm formula. Results: Group II has the largest mean inhibition with 76% compared to groups one and three. Conclusion: A combination of calcium hydroxide and propolis with a ratio of 1: 1.5 is more effective than 1:1 or 1:2 to inhibit A. actinomycetemcomitans biofilm. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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