| Autor: |
Timoshevskaya, Nataliya, Voss, S. Randal, Labianca, Caitlin N., High, Cassity R., Smith, Jeramiah J. |
| Předmět: |
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| Zdroj: |
Developmental Dynamics; Jun2021, Vol. 250 Issue 6, p822-837, 16p |
| Abstrakt: |
Background: Recent efforts to assemble and analyze the Ambystoma mexicanum genome have dramatically improved the potential to develop molecular tools and pursue genome‐wide analyses of genetic variation. Results: To better resolve the distribution and origins of genetic variation with A mexicanum, we compared DNA sequence data for two laboratory A mexicanum and one A tigrinum to identify 702 million high confidence polymorphisms distributed across the 32 Gb genome. While the wild‐caught A tigrinum was generally more polymorphic in a genome‐wide sense, several multi‐megabase regions were identified from A mexicanum genomes that were actually more polymorphic than A tigrinum. Analysis of polymorphism and repeat content reveals that these regions likely originated from the intentional hybridization of A mexicanum and A tigrinum that was used to introduce the albino mutation into laboratory stocks. Conclusions: Our findings show that axolotl genomes are variable with respect to introgressed DNA from a highly polymorphic species. It seems likely that other divergent regions will be discovered with additional sequencing of A mexicanum. This has practical implications for designing molecular probes and suggests a need to study A mexicanum phenotypic variation and genome evolution across the tiger salamander clade. Key Findings: Genome wide assessment of polymorphism in Ambystoma mexicanum and A. tigrinum.Identification of large A. tigrinum introgressed segments in laboratory stocks of A. mexicanumIdentification of transposable elements that expanded recently in A. tigrinum and mark large introgression regions, including recently active Habinger elements.tigrinum introgression intervals may harbor a disproportionately large number of biologically meaningful variants.This catalog of polymorphisms is an important resource for genomic/genetic studies and probe development in A. mexicanum. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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