| Autor: |
Numan, Abdulqawi, Al-Nedhary, Anass, Al-Hamadi, Mahfoudh, Saleh, Shaif, Ghaleb, Fares, Galil, Mansour |
| Předmět: |
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| Zdroj: |
Jordan Journal of Earth & Environmental Sciences; Mar2021, Vol. 12 Issue 1, p13-21, 9p |
| Abstrakt: |
The aim of the present work is to develop a novel, sensitive, fast, and cost-effective spectrophotometric method for the detection and quantitation of nitrite ions in vegetables. The method is based on the diazotization-coupling reaction of nitrite with solid sulfanilic acid and N-(1-naphthyl) ethylenediamine dihydrochloride in acidic medium. The final product is an azo dye with a maximum absorption at 542 nm. Variables such as amounts of solid diazo-coupling reagents, pH, and mass of charcoal, are optimized. The method detection and quantitation limits are respectively 0.010 mg L-1 and 0.030 mg L-1. The precession of the method reported as %RSD is below 5%, and the linear range is extended between 0.05 and 1 mg L-1 with R2 reaching 0.9999. The performance criteria of the proposed method under the most optimal conditions are validated and found to be superior compared to the performance of two selected reference methods and also to the results of the same method when diazo-coupling reagents are used as solutions. The proposed and validated method is used to quantitate nitrite in four leafy and root vegetables marketed in Yemeni. The method's recovery in the four tested vegetables is between 98.00% and 102.00% which is an indication of the high extraction efficiency and sensitivity of the developed method. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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