A Novel Approach to Rapid Determination ofβS-Globin Haplotypes: Sequencing of the Aγ-IVS-II Region.

Autor:	Vinson, Amy E., Walker, Aisha, Elam, Dedrey, Glendenning, Michele, Kutlar, Ferdane, Clair, Betsy, Harbin, Jeanette, Kutlar, Abdullah
Předmět:	GLOBIN genes HEMOGLOBINOPATHY GENETIC polymorphisms SICKLE cell anemia GENETIC mutation GENETICS
Zdroj:	Hemoglobin; Nov2004, Vol. 28 Issue 4, p317-323, 7p
Abstrakt:	Β-Globin gene cluster haplotypes were originally determined by restriction endonuclease mapping with Southern blots of polymorphic sites around the gene cluster. Over the years, haplotyping has been found to be useful, not only in population genetics but also in predicting the severity of hemoglobinopathies such as sickle cell disease. The sickle mutation occurs on five distinct haplotypes. The hitherto used methods are cumbersome and time-consuming, making haplotype determination a tedious procedure. We report our experience with a novel, rapid approach to haplotyping based on sequence polymorphisms in the Aγ-IVS-II region. We provide an algorithm that allows rapid assignment of the four African haplotypes carrying the sickle mutation. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
Externí odkaz:	Zobrazit plný text záznamu Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.