| Autor: |
Adhikari, Bhoj Raj, Yoshida, Koki, Paudel, Durga, Morikawa, Tetsuro, Uehara, Osamu, Sato, Jun, Muthumala, Malsantha, Amaratunga, Prasad, Arakawa, Toshiya, Chiba, Itsuo, Abiko, Yoshihiro |
| Předmět: |
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| Zdroj: |
Medical Molecular Morphology; Jun2021, Vol. 54 Issue 2, p79-86, 8p |
| Abstrakt: |
Oral cancer due to betel quid chewing habit is very common in South Asian countries. We attempted to detect the presence of a novel gene in epithelial cells stimulated with arecoline, a main component of betel quid. Human gingival epithelial progenitors were cultured and treated with a 3-day alternating regimen with/without 50 μg/ml arecoline for 1 month. DNA microarray and methylation arrays were analyzed to identify the candidate genes. Immunohistochemical staining was performed in the tissue samples. Genome-wide analyses, quantitative reverse transcription PCR and quantitative methylation-specific PCR revealed DUSP4 as the most significant and promising gene. The methylation levels of DUSP4 were significantly higher in the betel quid-related oral squamous cell carcinoma (OSCC) than those in the non-related OSCC and controls (Mann–Whitney U test, p < 0.05). The number of DUSP4 immunopositive cells in betel quid-related OSCC was significantly higher than those from the non-chewing patients and the controls (p < 0.05). Hypermethylation of DUSP4 may be considered as a specific event in betel quid-related oral cancer. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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