| Autor: |
Mikhalchik, E. V., Borodina, I. V., Vlasova, I. V., Vakhrusheva, T. V., Gorbunov, N. P., Panasenko, O. M., Titkova, S. M., Anurov, M. V., Ivakhov, G. B., Ermakov, I. V., Teplyshev, A. V., Klinov, D. V. |
| Zdroj: |
Biochemistry (Biokhimiya). Supplemental Series B, Biomedical Chemistry; Apr2021, Vol. 15 Issue 2, p125-131, 7p |
| Abstrakt: |
The progression of purulent inflammation in any acute surgical abdominal pathology can lead to local or diffuse peritonitis. The parameters characterizing the degree and specificity of the inflammatory response in blood such as cytokine concentration, neutrophil activity, plasma antioxidant capacity (thiols concentration) could be considered as potential predictors of complications. The luminol-dependent chemiluminescence (CL) response of blood activated by the phorbol ester (PMA), levels of cytokines IL-6, IL-8, IL-10, myeloperoxidase (MPO) and thiols were measured in plasma of patients with uncomplicated conditions (group 1, n = 8), local peritonitis (group 2, n = 9) or diffuse peritonitis (group 3, n = 9) at admission (before surgery, b/s), immediately after surgery (a/s) and a day after surgery (1 day) as well as in healthy volunteers (norm, n = 12). At all time-points the concentrations of cytokines and MPO measured by the ELISA method, in group 3 were higher than in healthy volunteers and in patients in groups 1 and 2. Blood CL demonstrated a more than 5-fold increase above the normal values in all patients, and it was also higher in group 2 as compared to group 1. In group 3 there were patients with both maximal and minimal CL values, which could be a consequence of neutrophil priming or exhaustion ("immune paralysis"), respectively. In these patients the plasma thiol content demonstrated a more than 3-fold decrease below normal values. In patients with fatal outcomes (group 3, n = 2) within a day after surgery, either a decrease of the CL to zero values concurrently with elevated IL-8 and IL-6 concentrations and low thiol levels was observed, or CL exceeded normal values more than 20 times with concurrent complete exhaustion of the plasma thiol pool. No clear dependency between the plasma parameters and neutrophil activity was found. This suggests that a panel of parameters for prognosis and/or early diagnostics of infectious complications in acute abdominal pathology should include different biomarkers of the inflammatory response: cytokine profile (IL-6, IL-8, IL-10), MPO and neutrophil activity, antioxidant plasma capacity (e.g., total thiols concentration). [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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