Autor: |
Lai, Catherine L., Campbell, Dianne E., Palmer, Debra J., Makrides, Maria, Santner‐Nanan, Brigitte, Gold, Michael, Tan, John Wei‐Liang, Valerio, Carol, Nanan, Ralph, Prescott, Susan L., Hsu, Peter S. |
Předmět: |
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Zdroj: |
Allergy; May2021, Vol. 76 Issue 5, p1385-1397, 13p |
Abstrakt: |
Background: Egg allergy affects almost 1 in 10 Australian infants. Early egg introduction has been associated with a reduced risk in developing egg allergy; however, the immune mechanisms underlying this protection remain unclear. Objective: To examine the role of regulatory immune cells in tolerance induction during early egg introduction. Methods: Cryopreserved peripheral blood mononuclear cells (PBMC) were obtained from infants from 2 randomized controlled trials of early introduction of egg for the primary prevention of egg allergy; BEAT (at 12 months, n = 42) and STEP (at 5 months n = 82; 12 months n = 82) study cohorts. In vitro ovalbumin‐stimulated PBMC were analyzed by flow cytometry for presence of ovalbumin‐specific regulatory T cells, using activation markers, FoxP3, and IL‐10 expression. Ovalbumin‐specific regulatory B cells were identified by co‐expression of fluorescence‐conjugated ovalbumin and IL‐10. Results: Specific, age‐dependent expansion of ovalbumin‐specific regulatory T cells was only observed in infants who (a) had early egg introduction and (b) did not have egg allergy at 12 months. This expansion was blunted or impaired in children who did not undergo early egg introduction and in those with clinical egg allergy at 12 months. Infants with egg allergy at 12 months of age also had reduced frequency of ovalbumin‐specific regulatory B cells compared to egg‐tolerant infants. Conclusion: Early egg introduction and clinical tolerance to egg were associated with expansion of ovalbumin‐specific T and B regulatory cells, which may be an important developmental process for tolerance acquisition to food allergens. [ABSTRACT FROM AUTHOR] |
Databáze: |
Complementary Index |
Externí odkaz: |
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