Construction of a linkage map based on aLathyrus sativusbackcross population and preliminary investigation of QTLs associated with resistance to ascochyta blight.

Autor: Skiba, B., Ford, R., Pang, E. C. K.
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Zdroj: Theoretical & Applied Genetics; Nov2004, Vol. 109 Issue 8, p1726-1735, 10p
Abstrakt: A linkage map of theLathyrus sativusgenome was constructed using 92 backcross individuals derived from a cross between an accession resistant (ATC 80878) to ascochyta blight caused byMycosphaerella pinodesand a susceptible accession (ATC 80407). A total of 64 markers were mapped on the backcross population, including 47 RAPD, seven sequence-tagged microsatellite site and 13 STS/CAPS markers. The map comprised nine linkage groups, covered a map distance of 803.1 cM, and the average spacing between markers was 15.8 cM. Quantitative trait loci (QTL) associated with ascochyta blight resistance were detected using single-point analysis and simple and composite interval mapping. The backcross population was evaluated for stem resistance in temperature-controlled growth room trials. One significant QTL, QTL1, was located on linkage group 1 and explained 12% of the phenotypic variation in the backcross population. A second suggestive QTL, QTL2, was detected on linkage group 2 and accounted for 9% of the trait variation. TheL. sativusR-QTL regions detected may be targeted for future intergenus transfer of the trait into accessions of the closely related speciesPisum sativum. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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