| Autor: |
NAM, ROBERT K., BENATAR, TANIA, AMEMIYA, YUTAKA, SETH, ARUN |
| Předmět: |
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| Zdroj: |
Cancer Genomics & Proteomics (1109-6535); May/Jun2021, Vol. 18 Issue 3, p197-206, 10p |
| Abstrakt: |
Background: We previously identified a panel of five miRNAs associated with prostate cancer recurrence and metastasis. Expression of one of the down-regulated miRNAs, miR-139-5p, was significantly associated with a lower incidence of biochemical recurrence and metastasis. Transcriptome profiling of miR-139-expressing prostate cancer cells revealed up-regulation of genes involved in interferon (IFN) stimulation. The association between miR-139 and IFN-ß was further explored in this study. Materials and Methods: We examined miR-139 transfected PC3, Du145 and LNCaP cells and the associated IFN response by transcriptome sequencing, immunoblotting and pulldown assays. Results: Treatment of prostate cancer cells by miR-139 resulted in the up-regulation of IFN-related genes. Specifically, miR-139 induced expression of the IFN-ß protein. The ability of miR-139 to induce IFN-ß was due to its binding to RIG-1 and the induction of IFNrelated genes was found to be dependent on RIG-1 expression. Conclusion: miR-139 acts as an immune agonist of RIG-1 to enhance IFN-ß response in prostate cancer cells. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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