Genistein Triggers Translocation of Estrogen Receptor-Alpha in Mitochondria to Induce Expressions of ATP Synthesis-Associated Genes and Improves Energy Production and Osteoblast Maturation.
| Autor: | Wu, Gong-Jhe, Cherng, Yih-Giun, Chen, Jui-Tai, Chang, Chuen-Chau, Liu, Shing-Hwa, Chen, Ruei-Ming |
|---|---|
| Předmět: |
ADENOSINE triphosphate
STATISTICS BONE growth ANALYSIS of variance ANIMAL experimentation MICROSCOPY BIOLOGICAL transport OSTEOBLASTS ESTROGEN receptors MITOCHONDRIA GENE expression RATS CELL survival IMMUNOBLOTTING T-test (Statistics) GENISTEIN RESEARCH funding MESSENGER RNA CONNECTIVE tissue cells POLYMERASE chain reaction DATA analysis |
| Zdroj: | American Journal of Chinese Medicine; 2021, Vol. 49 Issue 4, p901-923, 23p |
| Abstrakt: | Our previous study showed that estrogen can induce mitochondrial adenosine triphosphate (ATP) synthesis-associated gene expressions and osteoblast maturation. Genistein, a phytoestrogenic isoflavone that is widely found in various foods and traditional herb products, is beneficial for osteogenesis by selectively triggering estrogen receptor alpha (ER α) expression. In this study, we further investigated the mechanisms of genistein-induced energy production and osteoblast activation. Exposure of rat calvarial osteoblasts and human U-2 OS cells to genistein triggered osteoblast activation without affecting cell survival. Treatment with genistein time-dependently induced ER α mRNA and protein expressions in rat calvarial osteoblasts. Analyses by confocal microscopy and immunoblotting showed that genistein stimulated translocation of ER α from the cytoplasm to mitochondria. Subsequently, expressions of mitochondrial cytochrome c oxidase (COX) I and II mRNAs and proteins in primary rat osteoblasts were induced after exposure to genistein. Knocking-down ER α concurrently inhibited genistein-induced COX I and II mRNA expressions. In addition, mitochondrial complex enzyme activities, the mitochondrial membrane potential, and cellular ATP levels in rat calvarial osteoblasts were time-dependently augmented by genistein. Suppressing ER α expression instantaneously lowered genistein-induced enhancements of mitochondrial energy production and osteoblast activation. Effects of genistein on ER α translocation, COX I and II mRNA expressions, ATP synthesis, and osteoblast activation were further confirmed in human U-2 OS cells. This study showed that genistein can stimulate energy production and consequent osteoblast activation via inducing ER α -mediated mitochondrial ATP synthesis-linked gene expressions. [ABSTRACT FROM AUTHOR] |
| Databáze: | Complementary Index |
| Externí odkaz: |
|