| Autor: |
Beker, Merve, Uşak, Şule Terzioğlu, Boyraz, Rabia Kevser, Ünal, Shafiga Mursalova, Deveci, Erdem |
| Předmět: |
|
| Zdroj: |
Anatomy: International Journal of Experimental & Clinical Anatomy; 2020 Supplement, Vol. 14, pS112-S113, 2p |
| Abstrakt: |
Objective: Alzheimer's Disease (AD) is a severe neurological disorder resulting with neuronal death due to accumulation of amyloid beta (Ap) plaques. Beyond the approaches for clearance of plaques by pharmacological products, focused ultrasound (FUS) which is the focused beams of ultrasonic energy is a novel technology with a great potential of recovery for AD patients. The main object of this study was investigating the primary outcomes of FUS treatment on functional and molecular perspectives in a sporadic model of AD. Methods: 7 months old Long evans rats were infused with AJ3 to the hippocampus by using micro-osmotic pumps. Animals in FUS treatment group were sonicated in a targeted way under a stereotactic frame with a frequency of 2.8 MHz for 10 minutes during 20 successive days. Then animals were tested for memory functions by Morris water maze (MWAI) and passive avoidance (PA) behavioral tasks. Later, histological evaluation was made for neuronal survival through cresyl violet staining, for observing plaques through congo red staining and for neuronal degeneration through Fluoro Jade staining. On the molecular level, several important indicators of AD were also studied by western blot analysis. Results: Significant reduction in memory skills of Ap infused animals were observed in PA test. Also, number of surviving neurons in the hippocampus were found to be significantly lower in animals exposed to Ap, whether treated with FUS or not. There was no significant change in the number of degenerated neurons in the hippocampus among the groups. Protein expression studies indicated that, FUS treatment reduced the level of hippocampal pro-apoptotic Bax. Also, upregulated phosphorylation of Tau after Ap infusion was found to be reversed by FUS treatment. Conclusion: These results demonstrated that FUS is a promising treatment for AD through its molecular actions. However, long time observations and detailed functional tests are required for verifying our results. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
