| Autor: |
CHUN-SEOK YANG, IN-HWAN KIM, HYUN-DONG CHAE, DAE-DONG KIM, CHANG-HO JEON |
| Předmět: |
|
| Zdroj: |
In Vivo; May/Jun2021, Vol. 35 Issue 3, p1515-1520, 6p |
| Abstrakt: |
Background/Aim: The aim of this study was to detect circulating tumor cells (CTC) in the peripheral blood of gastrointestinal cancer patients using conditionally reprogrammed cell (CRC) culture. Materials and Methods: We confirmed the sensitivity of the CRC culture method. Five ml of blood were obtained from 81 cancer patients (56 colorectal and 25 gastric). The collected mononuclear cells were cultured for 4 weeks in the CRC condition. Finally, cultured cells were characterized by RT-PCR for the expression of hTERT and MAGE A1-6 mRNA. Results: The CRC method had a CTC detection limit of 6 cells for gastric cancer cells. After culture of 81 blood specimens, 38 formed visible cells, including 5 colonies. Among the 38 cells, 13 were hTERT positive and 4 were MAGE A1-6 positive. The final CTC detection rate was 16.0%. Conclusion: The CRC culture may potentially be used to evaluate the metastatic cancer cells in the circulation. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
