| Autor: |
K. P., Sharmila, Bhandary B., Satheesh Kumar, Kumari N., Suchetha, Bhat, Vadisha S., Fernandes, Ronald, Shetty, Jayaram, Peter, Alex John, Jose, Jerish M. |
| Předmět: |
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| Zdroj: |
Nitte University Journal of Health Science; 2018 Special Issue, p114-115, 2p |
| Abstrakt: |
Background: Radiotherapy is an important treatment modality for cancer and depends on the stage of the tumour, its localization and general health of the patient. However, effective use of ionizing radiation is compromised by the side effects that result from radiation-induced damage to normal tissue. The use of herbal radioprotectors and radioprotective compounds, which can selectively protect normal tissues against radiation injury, is of immense use as, in association with protecting the normal tissue, it will also permit use of higher doses of radiation to obtain better cancer control and possible cure. However, till date no ideal radioprotectors are available as most synthetic compounds are toxic at their optimal concentrations. Objective: The objective of the present investigation was to assess the level of Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), determine bone marrow cellularity and assess alpha esterase activity in swiss albino mice pretreated with asparagus racemosus root ethanolic extract (ARE) and Isoprinosine (IPR)compared to irradiated animals. Methodology: Swiss albino mice aged 6-8 weeks, weighing 20-25 g taken from an inbred colony were used for this study. The animals were divided into four groups of six animals each. Group I -- Control. Group II- radiation control, Group III- ARE-200 mg/kg body wt and Group IV- IPR-400 mg/kg body wt. The mice were fed with the ARE and IPR for 15 consecutive days prior exposure to sublethal dose(6Gy) of whole body electron beam radiation at a dose rate of 6Gy/min. The level of GM-CSF was assessed in the splenocytes using ELISA kit. Bone marrow cellularity was determined by trypan blue staining method using a hemocytometer and expressed as total live cells per femur. Azo dye coupling method was used to determine the non-specific α- esterase activity. The alpha (α)- esterase positive cells were scored microscopically. The data was analysed using ANOVA followed by Tukey's multiple comparison test. Result: Irradiationreduced the level of GM-CSF in the splenocytes which was enhanced by the pre-treatment with ARE and IPR.There was a drastic reduction in bone marrow cellularity in irradiated animals compared to normal control. Treatment with ARE and IPR significantly increased the bone marrow cellularity similar to controls. There was a significant increase in the number of alpha-esterase positive cells in the pre-treatment groups compared to radiation control. Conclusion: The present study demonstrates that AREand IPR exerts its radioprotection against Electron Beam radiation induced immunosuppression. This might be due to the phytoconstituents and increased antioxidant and free radical scavenging potential of Asparagus racemosus root ethanolic extract and Isoprinosine. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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