| Autor: |
Altaweel, Duaa A., Ahmed, Mohanad M., Abood, Haidar A. N. |
| Předmět: |
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| Zdroj: |
Biochemical & Cellular Archives; Apr2021, Vol. 21 Issue 1, p1777-1784, 8p |
| Abstrakt: |
Drug resistance continues to be a threat for usefulness of the antibiotic agents. combination therapy is a rational approach for those with risk factors for infections caused by ESBLs-Enterobacteriaceae since many have frequent â-lactamases resistance genes, which cause serious infections. The aim of the study was to assessment the efficacy of Levofloxacin, Amikacin, and Azithromycin combination to treat Enterobacteriaceae based on their phenotypic/genotypic background. A total of 30 multi drug resistances Enterobacteriaceae were collected from clinical isolates. Disc diffusion susceptibility method and VITEK2 were used for demonstrated the phenotypic characteristics of the isolates. Polymerase chain reaction (PCR) targeting ESBLs (bla-CTXMI, bla- SHV, and bla-TEM) was used for the molecular genotyping of resistance genes. Checkerboard was used to evaluate the synergism of triple antibiotics combination (levofloxacin, amikacin and azithromycin). Our results were revealed that the prevalence of ESBLs resistance genes were (86.6%) and the predominant resistance enterobacterial was Kl. pneumonia (46.7%). The triple combination is significantly correlated with bla- TEM, with 33% synergistic interaction and 3% additives. In conclusion, In vitro data suggest that triple antibiotics combination (levofloxacin, amikacin and azithromycin) can be effective even if the bacteria are resistant to the individual antibiotics, that can used to treat infections caused by multi resistance Enterobacteriaceae. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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