| Autor: |
Khudhair, Zainab Yaseen, Jameel, Zahraa J., Ammari, Abbas |
| Předmět: |
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| Zdroj: |
Biochemical & Cellular Archives; Apr2021, Vol. 21 Issue 1, p187-190, 4p |
| Abstrakt: |
Twenty-nine cutaneous sample, (Baquba Teaching and Al Khalis Hospital) during the period from12/ 1/2019 to the2/2/ 2020/end of February with (ranging from 1-11) years old according the gender with dermatology (male 14and female 16), the inhibition effect of the pyocyanin stain was tested against the isolates of Candida resistant to antifungal agents. The antibiotic sensitivity test was (amphotericin B, ketoconazole). The results showed that the isolates were resistant to ketoconazole by 75% and the percentage of amphotericin B was 33.3%. The pyocyanin stain, transformed by Pseudomonas aeruginosa, is a secondary metabolite seen to have an important role in bacterial activity and competition in the environment. The results indicated that the pyocyanin extract isolated from clinical and environment sources (Burns, blood, urine and middle ear) at a concentration of (75%, 100%, 50% and 25%). It has a fatal effect for candida as for the isolated blood and soil source; it has no effect against Candida. The DNA of the Candida albicans yeast isolates responsible for the genes for the virulence factor were extracted by specific primers namely, AINT1 using polymerase chain reaction (PCR) and gene size detection showed the result of electrical gel product that11 (90%) the isolate were positive bearing gene AINT1. PCR product was sent to gene to find out the nucleotide sequence and mutations present in both genes using the Gene-Sequencing technique. The results of Sequence analysis for gene after the corresponding procedure showed that the isolates treated with the source of pyocyanin for the AINT1 gene from (1-4) were identical. Conclusions Detection of the AINT1 gene dermal samples is 72% higher [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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