| Abstrakt: |
Polymers are considered in two basic classes as natural (polymers produced by biological-systems) and synthetic (chemically synthesized from starting materials). They are widely used in food, textile, pharmaceutical and biomedical industries. Although synthetic polymers have a widespread use, the uses of natural polymers is increasing day by day. Biopolymers in class of natural-polymers are naturally occurring polymers in the life process of living-organisms. They are obtained from biomass and natural and/or genetically modified organisms. Their synthesis is usually based on enzyme-catalyzed-reactions and chain-growth from activated monomers by complex metabolic processes within cells. They are light, inexpensive, easy-shape, excellent mechanical-properties, chemically inert and corrosion-resistant macromolecules. In this respect, biopolymers have become more preferred in recent years, especially in biomimetic-approaches in field of tissueengineering. Polymeric biomaterials, which are identical to geometrically damaged tissue for tissue regeneration and serve as matrix to which cells can adhere, are used as scaffold. Biopolymers can be synthesized commercially in reactive purity and, in addition can be obtained by isolation from natural sources under laboratory conditions. Commonly used in biomaterial production; alginic acid is obtained from brown-red algae, while collagen is generally obtained from tendons of bovine/ovine-animals, rat-tail tendons and the like. The aim of this study was to isolate sodium alginate from brown-sea algae, and collagen from rat-tail tendons and purify them for ready to use in cell-culture. The scaffolding-formation potential of the biopolymericstructures by lyophilization was also investigated. As a result, it was found that sodium-salt of alginicacid can be obtained with high purity by step-fractionation. Collagen was obtained as type-1-collagen molecule from the tendons, and had been purified by dissolving and filtered tendons in acidic solution. In conclusion, the biodegradability analysis of lyophilized collagen and alginate scaffolds in HANK's solution showed that scaffolds have durability to allow new tissue formation for tissue engineering applications. [ABSTRACT FROM AUTHOR] |
