Crystallographic study of a naturally occurring trans-splicing intein from Synechocystis sp. PCC 6803.

Autor: Chen, Xuehui, Xu, Ming-Qun, Ding, Yi, Ferrandon, Sébastien, Rao, Zihe
Předmět:
Zdroj: Acta Crystallographica: Section D (Wiley-Blackwell); Jul2002, Vol. 58 Issue 7, p1204-1206, 3p
Abstrakt: A naturally occurring split intein from the dnaE gene of Synechocystis sp. PCC6803 (Ssp DnaE intein) has been purified and crystallized using PEG 8K as precipitant. The crystal belongs to space group P3121 or P3221, with unit-cell parameters a = b = 58.5, c = 70.2 Å. it has one molecule per asymmetric unit and diffracts to beyond 2.9 Å under cryoconditions (110 K) using a Cu rotating-anode X-ray generator in-house. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index