| Autor: |
García-Palencia, Patricia, Rivas-Morales, Catalina, Oranday-Cárdenas, Azucena, Angélica de la Garza-Ramos, Myriam, Leos-Rivas, Catalina, Torres López, Ernesto |
| Předmět: |
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| Zdroj: |
Journal of Medicinal Plants & By-Products; 2021 Special Issue, Vol. 10, p51-59, 9p |
| Abstrakt: |
Periodontal diseases are characterized by inflammation and destruction of tooth-supporting tissues. There is interest in antiinflammatory substances of plant origin because of their advantage in relation to classic anti-inflammatory drugs. The cytotoxic and anti-inflammatory effect of extracts of Scopulophila parryi and Psacalium decompositum (A.Gray) H.Rob. & Brettell were evaluated with the 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium (MTT) method and their proinflammatory cytokine profile was determined in a human peripheral blood monocyte culture model. The plant was collected and identified and the methanol extract was prepared by maceration. Cytotoxicity was evaluated in vitro with the MTT (3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium) assay in two cell lines, VERO and Detroit 551 human fibroblasts. The anti-inflammatory activity of human peripheral blood monocytes and gene expression of the proinflammatory cytokines, IL-1, IL-6 and IL-8, were evaluated The extract of P. decompositum was less toxic on the VERO and Detroit 551 cell lines with a DL50 of 77.30 µg/mL and the S. parryi extract had 150.58 µg/mL. Both extracts significantly reduced expression of IL-1ß, IL-6, and IL-8 at 300 µg/mL for S. parryi and at 400 µg/mL for P. decompositum. The extract of P. decompositum was less toxic for both cell lines and the two methanol extracts are good alternatives as immunomodulators of proinflammatory cytokines. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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