| Autor: |
ZONGLIANG YANG, TIAN NIE, TIANSHU PENG, JIAN YI, HAIYAN LU, SHENGYAN XU, XIAOBING FU, XIANGDANG HU |
| Předmět: |
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| Zdroj: |
Acta Poloniae Pharmaceutica; Jan/Feb2021, Vol. 78 Issue 1, p49-54, 6p |
| Abstrakt: |
Colon cancer is one of the leading cancer types that positioned the 3rd rank in the global level. The role of CXCR2 and CD133 is reported in many different types of cancers. The aim of the present investigation is to study the relation between miRNA-92a in association with CXCR2 and CD133. Azoxymethane/Dextran sulfate sodium combination was used to develop initial and advanced stage colon cancer. Histology, immunohistochemistry, in-situ hybridization, and Western blotting techniques are used to differentiate cancer stages and aid in analyzing CXCR2, CD133, and miRNA-92a expression. The mice develop the initial and advanced stage of colon cancer following a one-time injection of Azoxymethane followed by 4 times or 6 times Dextran sulfate sodium injection in every 5 days interval. Histology confirms moderate differentiation of cells and cellular loci formation in initial colon stages, but advanced stages are evident with more clumps of undifferentiated cells which form multiple loci. The expression of CXCR2, CD133, and miRNA-92a is showing a similar pattern of expression that it is upregulated to certain levels in initial colon cancer stages but it is abruptly overexpressed in advanced stages of colon cancer. The result concludes that the expression of miRNA-92a regulates CD133, which is associated with colon cancer stem cells that favor the progression of colon cancer along with CXCR2 overexpression. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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