Laboratory Testing of a New Strain of the Insect-pathogenic Fungus Metarhizium anisopliae "S54" as a Potential Control Agent for Lygus keltoni (Hemiptera: Miridae), A Pest of Canola and Alfalfa in Western Canada.

Autor: XUE DONG CHEN, JOHNSON, DAN L., CÁRCAMO, HÉCTOR, JEGATHEESWARAN, PIRATHEEPA, KAWCHUK, LARRY, JARONSKI, STEFAN
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Zdroj: Biopesticides International; Jul-Dec2020, Vol. 16 Issue 2, p101-110, 10p
Abstrakt: An entomopathogenic fungus with biopesticide potential, Metarhizium anisopliae sensu lato "S54," also called train "DJ1" by the USDA, was isolated from soil in southern Alberta, Canada. In vitro conidial germination rate, mycelial growth, and colony size were examined under different heat stress conditions. The susceptibility of Lygus keltoni (Hemiptera: Miridae) to this isolate was tested under laboratory conditions. Bioassays were performed to evaluate the lethal concentration (LC50) and average survival time (AST) of the isolate against L. keltoni. Dose-response mortality bioassays using several concentrations of M. anisopliae indicated LC50 values of 6.3 x 104 conidia/mL at 7 days after inoculation with L. keltoni. The AST was 4.32-6.52 days. The results demonstrate that strain S54 performed well in terms of radial growth, conidial yield, and rate of conidial germination at 35°C. Furthermore, conidia continued to grow vigorously for 10 days after an 8 h exposure to 48°C. The role of such a high-temperature tolerant strain in crop protection is discussed for its potential to lessen our reliance on insecticides to reduce yield losses. A biopesticide based on M. anisopliae has significant potential to manage L. keltoni and other plant bugs in alfalfa and canola. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index